Biochemical and Histochemical Study on Fatty Acid-Calcium Gallstones

Histochemical study on orotic acid.

Recently orotic acid has been noticed as a growth factor and regarded as a pre cursor of nucleic acid (1, 2). But its distribution in organisms has scarcely been studied except for tracing the acid using C14-labeled compound (3). The authors have found that orotic acid shows a whitish blue primary fluorescence, which is re markably intensified by adding H2O2. Applying this property to histochem...

[Histochemical and biochemical studies in acute tannic acid poisoning].

Mechanisms of fatty acid effects on sarcoplasmic reticulum. I. Calcium-fatty acid interaction.

To elucidate the mechanism for the previously reported increase in calcium sequestration by sarcoplasmic reticulum vesicles in the presence of palmitic acid, the ability of this fatty acid to bind calcium was investigated by dual wavelength spectrophotometry using the calcium indicator arsenazo III,, and by Millipore filtration using 45CaCl2. In the presence of 120 mM KCl, 1 mM MgCl2, 15 microM...

Histochemical and biochemical studies on leukocyte alkaline phosphatase activity.

Histochemical study on thiamine.

The authors have applied the thiochrome method for determining thiamine to histochemical investigations and could observe the localization of thiamine in cells and tissues (1-6). Independently of our study, v. Muralt (7) had already reported a investigation on histological pictures of thiamine in peri pheral nerves by the thiochrome method, which was however unavailable to the authors at that t...